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将胎龄为17d的大鼠胚胎海马(移植物)移植到同种成年大鼠(宿主)腹海马,90d后,用TH免疫细胞化学方法,对海马移植物内神经元与儿茶酚胺(catecholamineCA)能纤维之间的突触联系进行了观察。免疫组织化学显示,宿主海马内有大量TH免疫反应纤维,这些纤维是直径为0.5—1μum的细纤维。它们的分布密度是,齿回门区和CA3区透明层内较密集,分子层次之,锥体细胞层和颗粒细胞层内较稀疏。在移植物的分子层和细胞层内,也有TH免疫反应纤维,它们略粗,有些纤维的直径大于1μm,纤维末端分支较多.免疫电镜的观察,在海马移植物内,看到许多TH免疫反应终扣与非免疫反应的树突棘和树突形成突触联系,这些突触主要是非对称型突触,突触间隙约30nm左右,突触后膜明显增厚。以上结果表明:宿主脑内的CA能神经纤维可能是通过侧校长芽的形式,向移植物投射。投射到移植物内的CA能纤维,能同移植物内的神经细胞建立突触联系。
The hippocampus of rat embryo age 17d was transplanted into the hippocampus of the same kind of adult rat (host). After 90 days, TH immunocytochemistry was used to evaluate the effects of catecholamine CA Synaptic connections between fibers were observed. Immunohistochemistry showed that the host hippocampus has a large number of TH immune response fibers, these fibers are 0.5-1μum in diameter of fine fibers. Their distribution density, the tooth back door and CA3 area transparent layer more dense, molecular level, pyramidal cell layer and granular cell layer more sparse. Within the molecular and cellular layers of the graft, there are also TH-immunoreactive fibers, which are slightly thicker, with some of the fibers having a diameter of more than 1 μm and more fiber-end branches. Immunoelectron microscopy showed that many TH immune responses in the hippocampal grafts were seen to form synaptic connections with non-immunoreactive dendritic spines and dendrites. These synapses are mainly asymmetric synapses with synaptic cleft about 30 nm Around the postsynaptic membrane was significantly thicker. The above results show that CA nerve fibers in the host brain may be projected onto the graft through the lateral principal buds. The CA fibers that project into the graft build synaptic connections with the nerve cells in the graft.