IL-1是一类具有相似生物学功能的蛋白质家族,其中IL-1α和IL-1β已经鉴定和纯化.目前已有一些方法用于测定IL-1,生物检测法用于测定分泌的总IL-1,免疫检测法用于测定分泌的IL-1β.最近,利用免疫细胞化学法和免疫荧光显微定量法检测IL-1β和IL-1α.但是,免疫荧光显微定量法易受主观因素的影响而且费时.本文利用流式细 IL-1 is a family of proteins with similar biological functions, among which IL-1α and IL-1β have been identified and purified.At present, some methods have been used to determine IL-1 and bioassay to determine the total secreted IL 1, an immunoassay for the measurement of secreted IL-1 [beta]. Recently, immunocytochemistry and immunofluorescence microscopy have been used to detect IL-1 [beta] and IL-I [alpha]. However, immunofluorescence microscopy is susceptible to subjective factors The impact and time-consuming.This paper uses the flow fine