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目的：研究真核生物水稻的苯丙氨氨氨解酶基因在原核生物大肠杆菌中表达及其规律，为酶替代疗法治疗苯丙酮尿症及某些肿瘤治疗奠定基础。方法：根据水稻苯丙氨酸氨解酶基因序列，在ＡＴＧ下游１３１－１１２碱基处设计引物：５’ＴＧＧＡＴＣＴＴＧＡＣＣＡＧＣＧＧＣＴ３’?
OBJECTIVE: To study the expression and regulation of the phenylalanine aminotransferase gene in prokaryotic E. coli in eukaryotic rice, and to lay the foundation for the treatment of phenylketonuria by enzyme replacement therapy and the treatment of certain tumors. Methods: According to the sequence of rice phenylalanine ammonia-lyase gene, primers were designed at 131-112 bases downstream of ATG: 5’TG-GATCTTGACCAGCGGCT3 ’