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报道了Ｈｅ—Ｎｅ激光对白细胞ＤＮＡ诱变的拉曼光谱研究。Ｈｅ—Ｎｅ激光辐射前，白细胞ＤＮＡ主链振动区域出现二条强的Ａ，Ｃ构型特征线８１２ｃｍ－１和８７２ｃｍ－１，以及弱的Ｂ型语线１０９２ｃｍ－１。辐射后，Ａ型特征线８１２ｃｍ－１消失，代之是与Ｂ型结构有关的二条强的语线８００ｃｍ－１和１０９０ｃｍ－１。试验结果表明，Ｈｅ—Ｎｅ激光可诱变ＤＮＡ构型（ＡＭ－Ｂ型）。在碱基振动区域，辐射前其拉曼谱出现强而宽的Ｃ＝０伸长振动谱线１６８０ｃｍ－１，这是与酮基结构有关的谱线，辐射后该谱线强度大大减弱。实验结果提示，Ｈｅ一Ｎｅ激光有可能使碱基发生互变异构。
A Raman spectrum study of white blood cell DNA mutagenesis by He-Ne laser was reported. Before He-Ne laser irradiation, two strong A and C configuration characteristic lines 812cm-1 and 872cm-1 and a weak B-type line 1092cm-1 appeared in the vibrational region of the white blood cell DNA. After radiation, type A characteristic line 812cm-1 disappears, instead of the B-type structure of the two strong language lines 800cm-1 and 1090cm-1. The experimental results show that He-Ne laser can mutate DNA configuration (AM-B type). In the vibrational region of the base, its Raman spectrum appears strong and wide before the irradiation, the vibrational line of C = 0 stretching vibration is 1680cm-1, which is the line related to the structure of the keto group. The intensity of this line is greatly weakened after irradiation. Experimental results suggest that He-Ne laser may cause base tautomerism.