冰冻切片制片技术的优劣不同程度地影响快速病理诊断水平。我们在工作中发现,切出的冰冻切片略微吹干后,经30s福尔马林处理及5s乙醇处理,再进行HE染色,其镜下观察效果与石蜡切片较为接近。 1 材料和方法 半导体冰冻切片机,HE染色液及福尔马林等。对下述三种方法制得的冰冻切片进行比较。 1.1 新鲜组织置冰冻切片机切片,贴于载玻 The advantages and disadvantages of frozen section preparation technology to varying degrees affect the rapid pathological diagnosis. We found at work that the sliced ​​frozen slices were slightly dried and treated with formalin for 30s and ethanol for 5s, then stained with HE. The microscopic observation was similar to that of paraffin sections. 1 Materials and Methods Semiconductor frozen section machine, HE stain and formalin and so on. Frozen sections prepared by the following three methods were compared. 1.1 fresh tissue frozen section machine slice, affixed to the glass