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本文研究单唾液酸神经节苷脂（ＧＭ１）对大鼠中脑多巴胺（ＤＡ）神经元体外生存及其损伤后的保护作用。取胚胎腹侧中脑制成细胞悬液，常规体外培养。实验分为四组：第一组在培养液中加入ＧＭ１（ＧＭ１组）；第二组在培养过程中使用１－甲基－４－苯基－１，２，３，６－四氢吡啶（ＭＰＴＰ）破坏ＤＡ神经元（ＭＰＴＰ组）；第三组在使用ＭＰＴＰ破坏ＤＡ神经元的同时给予ＧＭ１（ＧＭ１－ＭＰＴＰ组）；第四组为正常对照组。四组培养细胞定期抽出作免疫组化和荧光组化染色。ＧＭ１组培养各阶段，酪氨酸羟化酶（ＴＨ）免疫反应阳性细胞数量均明显多于对照组，至体外培养２周时，每孔阳性细胞数平均可达７２．８个，与对照组相比有显著性差异。ＭＰＴＰ组在加入ＭＰＴＰ１周左右，荧光组化阳性细胞已基本消失，此后ＴＨ免疫反应阳性细胞逐渐减少，至体外培养２周，每孔ＴＨ阳性细胞数平均仅剩１４．５个，与对照组相比有显著性差异。在残存的ＤＡ神经元中，突起变短或消失。ＧＭ１－ＭＰＴＰ组，体外培养期间均可看到荧光组化阳性细胞，ＴＨ免疫反应阳性细胞数量在体外培养２周时平均每孔为３１．７个，明显高于ＭＰＴＰ组。研究结果表明ＧＭ１能提高体外生长的ＤＡ神经元的生存率，并能减轻ＭＰＴＰ对?
This study was designed to investigate the protective effect of monosialoganglioside (GM1) on the survival and injury of dopamine (DA) neurons in rat midbrain in vitro. Take the embryonic ventral midbrain cell suspension, routine in vitro culture. The experiment was divided into four groups: the first group added GM1 (GM1 group) to the culture medium; the second group used 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP group). The third group was given GM1 (GM1-MPTP group) while MPTP was used to destroy DA neurons. The fourth group was normal control group. Four groups of cultured cells were regularly extracted for immunohistochemistry and fluorescence staining. The number of positive tyrosine hydroxylase (TH) immunoreactive cells in GM1 group was significantly higher than that in control group at each stage of culture. When cultured in vitro for 2 weeks, the average number of positive cells per well reached 72.8, Compared to significant differences. MPTP group at about 1 week after the addition of MPTP, fluorescence-positive cells have disappeared, then TH immunoreactive positive cells gradually decreased to 2 weeks in vitro, the average number of TH positive cells per well only an average of 14.5, with the control group phase Than the significant difference. In remnants of DA neurons, the protrusions become shorter or disappear. GM1-MPTP group. Fluorescence-positive cells could be seen during in vitro culture. The number of TH-immunoreactive cells in each group was 31.7 per well at 2 weeks in vitro, which was significantly higher than that in MPTP group. The results show that GM1 can increase the survival rate of DA neurons grown in vitro, and can reduce the MPTP on?