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未成熟心肌细胞的培养是以新生动物和胚胎的心肌组织作为实验材料,采用酶消化法在体外培养建立的一种实验模型,该模型建立的关键在于如何提高心肌细胞的存活率、搏动率和纯化率,在电生理、中药、心血管疾病研究领域已显示其重要意义。通过检索国内外相关文献,对未成熟心肌细胞的培养方法进行着重阐述,并结合心肌细胞的形态生理学、鉴定方法、培养过程中的影响因素和应用等方面进行综合分析。
The immature cardiomyocyte culture is an experimental model established by in vitro culture of neonatal animal and embryonic myocardium using the enzyme digestion method. The key point of this model is how to improve the survival rate of cardiomyocytes, the rate of pulsation and Purification rate, electrophysiology, traditional Chinese medicine, cardiovascular disease research has shown its significance. Through the retrieval of relevant literatures at home and abroad, the methods of culturing immature cardiomyocytes are emphatically described, and combined with the morphological physiology of cardiomyocytes, identification methods, the influencing factors in the culture process and its application.