化学物质诱发实验性肝癌,过去多从整体水平也即体内诱变。这一方法,由于体内环境错综复杂,影响因素和作用环节较多,不便分析结果。即便体外诱变,除极个别报道外,绝大多数为原代培养物。这些材料,由于所获细胞不够纯一、存活时间较短、传代困难,因此限制了使用范围和价值。另一方面,已知甲胎蛋白(AFP)是哺乳类胚胎期出现于血清的一种蛋白质,它与肝癌的关系十分密切。成年期一旦发生肝癌,那么已降至低水平的AFP量又复上升,可达到胚胎期的高水平程度。因此,AFP的变动可用作判别实验诱变的一个有效指征。鉴于上述,我们新建立了一个能够反映AFP动态变化的正常新生大鼠肝细胞系NRL- Chemical induced liver cancer, in the past from the overall level that is, in vivo mutagenesis. This method, due to the intricate body environment, factors and the impact of more links, inconvenient to analyze the results. Even in vitro mutagenesis, with the exception of very few reported, the vast majority of primary cultures. These materials, because the cells obtained are not pure enough, survive for a short time, passaging difficulties, thus limiting the scope and value of use. On the other hand, alpha-fetoprotein (AFP) is known to be a protein found in the serum of mammalian embryos and has a close relationship with liver cancer. Once the occurrence of liver cancer in adulthood, then the amount of AFP has been reduced to a low level and rise again, reaching a high level of embryonic stage. Therefore, AFP changes can be used as a valid indicator of experimental mutagenesis. In view of the above, we have newly established a normal neonatal rat liver cell line that can reflect the dynamic changes of AFP NRL-