论文部分内容阅读
本研究用电镜(辅以半薄切片的光镜检查)观察了3-氯丙二醇及2,3-氧丙醇合并用药对大白鼠附睾起始部及尾部超微结构的影响。同时,对二者单独使用时的作用进行了对比观察。结果表明:在较低有效抗生育剂量合并用药(α-ch 5mg+gly 75mg/kg/天×2)3天后,多数动物附睾管的主细胞、顶端细胞、基底细胞及亮细胞,管壁平滑肌细胞以及间质微血管内皮细胞的线粒体出现了不同程度的肿胀和退变。部分动物附睾上皮主细胞的高尔基大泡,吞饮小泡、多泡小体、不动纤毛及顶部滑面内质网亦有减少。细胞核未见明显改变。随着停药时间的延长,正常形态的线粒体的数量有相对增加的趋向,其它因药物作用而变化的细胞器亦趋向恢复。在高剂量合并用药组(α-ch 20mg+gly 100 mg/kg/天×2),除见有上述变化外,在部分动物的少数附睾管断面见到上皮细胞坏死、脱落,精子的质膜破裂、线粒体肿胀及核固缩等改变。单独使用3-氯丙二醇及2,3-氧丙醇对附睾超微结构的影响与合并用药相似。但二者影响的程度不及合并用药明显。这类药物在较低有效抗生育剂量下引起的附睾上皮细胞线粒体的损伤,主细胞高尔基复合体、滑面内质网及吞饮小泡的减少或功能降低,可能导致附睾正常生理功能的改变,从而造成不利于精子成熟的环境而产生抗生育作用。此外,药物对附睾管壁平滑肌细胞的线粒体及附睾微血管内皮细胞线粒体均引起不同程度的损伤,其程度严重者可能导致精液囊肿的产生。
In this study, the effects of 3-chloropropanediol and 2,3-oxopropanol on the ultrastructure of the epididymis and the tail of the epididymis were investigated by electron microscopy (assisted by light microscopy of semi-thin sections). At the same time, the effects of the two when used alone were compared. The results showed that the main cells, apical cells, basal cells, and bright cells of the epididymal ducts of most animals after 3 days of low effective antifertility doses (α-ch 5mg+gly 75mg/kg/day×2), smooth muscle wall Mitochondria of cells and interstitial microvascular endothelial cells showed different degrees of swelling and degeneration. In some animal epididymal epithelial cells, the Golgi’s large vesicles, swallowed vesicles, multivesicular bodies, immobile cilia, and endoplasmic reticulum at the top of the epidermis were also reduced. No significant changes in the nucleus. With the prolonged drug withdrawal time, the number of normal mitochondria tends to increase, and other organelles that change due to drug action tend to recover. In the high-dose combination group (α-ch 20mg+gly 100 mg/kg/day×2), in addition to the above changes, epithelial cell necrosis and shedding were observed in a small number of epididymal ducts in some animals. Rupture, mitochondrial swelling, and nuclear condensation change. The effects of 3-chloropropanediol and 2,3-oxopropanol alone on epididymal ultrastructure were similar to those of combined use. However, the degree of influence of the two is less than that of the combined medication. The reduction of mitochondria in the epididymal epithelial cells induced by the lower effective antifertility dose, the decrease or function of the main cell Golgi complex, the smooth endoplasmic reticulum, and the phagocytic vesicles may lead to changes in the normal physiological functions of the epididymis. , resulting in an environment that is not conducive to the maturation of spermatozoa, resulting in anti-fertility effects. In addition, mitochondria of epididymal smooth muscle cells and mitochondria of epididymal microvascular endothelial cells were caused to damage in different degrees by the drug. Severe cases may lead to the production of semen cysts.