SV_(40)即猴乳多空病毒,是猴的一种潜在病毒。SV_(40)对恒河猴的隐性感染,可致原代恒河猴肾组织培养细胞(PRMK)的严重污染,从而影响用PRMK制备的疫苗质量及安全性。目前,国内许多实验室还难以检查PRMK中的SV_(40),故使用电镜检查SV_(40)具有实际价值。本文报告国内使用电镜首次检查到SV_(40)。对所分离到的4株病毒进行阴性染色制样,同时用国际标准SV_(40)及 SV_ (40) is the papaya virus and is a potential monkey virus. Recessive infection of rhesus monkeys with SV_ (40) can cause severe contamination of primary rhesus monkey kidney tissue culture cells (PRMK), affecting the quality and safety of vaccines prepared with PRMK. At present, it is hard to check the SV_ (40) in PRMK in many laboratories in our country. Therefore, it is of practical value to inspect SV_ (40) by electron microscopy. This paper reports the first inspection of SV_ (40) using electron microscopy in China. The isolated 4 strains were negative stain samples, while using the international standard SV_ (40) and