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应用免疫组化技术(ABC法)回顾性研究1980～1988期间21例神经母细胞性肿瘤,其中神经母细胞瘤12例,节细胞神经母细胞瘤:分化差性2例,混合性4例,分化性3例。结果显示阳性病例NSE17例(80.95％),S-100 15例(71.43％),GFAP 6例(28.57％)。阳性病例数在各组织类型中随瘤组织分化程度而增加。其免疫标记染色强度与瘤细胞分化程度基本一致。研究表明NSE在新生儿和儿童的小细胞性肿瘤仍可用作诊断神经母细胞瘤较特异的标记。S-100在周围神经组织和有关组织与肿瘤则表达了神经鞘或神经鞘样细胞的分化,与节细胞分化一样,可认为是神经母细胞瘤组织学成熟的标志。其数量也可作为神经母细胞性肿瘤分型判别的根据,具有预后意义。GFAP在本组中分布与S-100相同,但阳性率低下,可作为周围神经及其相关组织和肿瘤的神经鞘或神经鞘样细胞分化的辅助标志。一些瘤细胞出现抗原表达的变异或多种抗原是与该瘤细胞的分化过程变异有关。
21 cases of neuroblastoma, including neuroblastoma, 12 cases, ganglioneuroblastoma: poorly differentiated in 2 cases, mixed in 4 cases, neuroblastoma in 21 cases, were retrospectively studied by immunohistochemistry (ABC method) Differentiation in 3 cases. The results showed that positive cases of NSE17 cases (80.95%), S-100 in 15 cases (71.43%), GFAP in 6 cases (28.57%). The number of positive cases increased with the degree of tumor differentiation in each tissue type. The immunostaining staining intensity and tumor cell differentiation basically the same. Studies have shown that small cell tumors of NSE in neonates and children can still be used as a more specific marker for the diagnosis of neuroblastoma. S-100 expression of nerve sheath or nerve sheath-like cells in peripheral nerve tissue and related tissues and tumors, like the differentiation of ganglion cells, can be considered as a sign of histological maturation of neuroblastoma. Its number can also be used as a basis for the classification of neuroblastic tumor, with prognostic significance. GFAP in the group distribution and S-100 the same, but the low positive rate, can be used as peripheral nerve and its related tissues and tumors of nerve sheath or nerve sheath-like cells auxiliary markers. Some tumor cells appear antigenic variation or antigen expression is related to the process of tumor cell differentiation process variation.