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目的探讨外源性给予骨髓间充质干细胞(MSCs)与CD133+肾脏细胞对缺血再灌注(I/R)诱导的急性肾损伤小鼠模型的保护作用。方法雄性C57BL/6小鼠32只,分为正常对照组、I/R组、I/R+MSCs组、I/R+CD133+肾脏细胞组,每组8只。在各组模型建立后的第1、2、3、7天分别处死2只。获取动脉血,检测其血尿素氮(BUN)与肌酐(Cr)水平;切取肾组织,观察肾组织的病理改变,并对受损肾组织行急性肾小管坏死程度(ATN)评分。应用酶联免疫(ELISA)法检测肾组织匀浆中肿瘤坏死因子-α(TNF-α)、肝细胞生长因子(HGF)的水平。结果①I/R+MSCs组术后BUN、Cr水平,ATN评分及TNF-α水平高于正常对照组,而低于I/R组,以术后第7天的差异最为显著,P<0.05。I/R+MSCs组术后HGF水平低于正常对照组,高于I/R组,以术后第7天的差异最为显著,P<0.05。②I/R+CD133+肾脏细胞组的术后BUN、Cr水平,ATN评分及TNF-α水平高于正常对照组,而低于I/R组,以术后第3天的差异最为显著,P<0.01。HGF的检测结果与之相反,I/R+CD133+肾脏细胞组术后HGF水平低于正常对照组,高于I/R组,以术后第3天的差异最为显著(P<0.01)。③I/R+CD133+肾脏细胞组与I/R+MSCs组比较,其术后BUN、Cr水平,ATN评分及TNF-α水平较低,HGF水平较高(P<0.05)。结论 MSCs与CD133+肾脏细胞可能通过内分泌方式调控细胞因子,改善微环境,促进由I/R诱导的急性肾损伤恢复,而CD133+肾脏细胞对急性肾损伤的修复作用更为明显。
Objective To investigate the protective effects of exogenous administration of bone marrow mesenchymal stem cells (MSCs) and CD133 + kidney cells on acute kidney injury induced by ischemia / reperfusion (I / R) in mice. Methods Thirty-two male C57BL / 6 mice were divided into normal control group, I / R group, I / R + MSCs group and I / R + CD133 + renal cell group. Two rabbits were sacrificed on the 1st, 2nd, 3rd and seventh days after the model was established. Arterial blood was obtained and blood urea nitrogen (BUN) and creatinine (Cr) levels were measured. The kidneys were dissected and the pathological changes of renal tissues were observed. The degree of acute tubular necrosis (ATN) was evaluated in injured renal tissues. The levels of tumor necrosis factor-α (TNF-α) and hepatocyte growth factor (HGF) in kidney homogenates were detected by enzyme linked immunosorbent assay (ELISA). Results ① The levels of BUN, Cr, ATN and TNF-α in I / R + MSCs group were significantly higher than those in normal control group, but lower than those in I / R group (P <0.05). The level of HGF in postoperative I / R + MSCs group was lower than that in normal control group, but higher than that in I / R group (P <0.05). ② The levels of BUN, Cr, ATN and TNF-α in I / R + CD133 + kidney cell group were higher than those in normal control group, but lower than those in I / R group, the difference was most significant on the 3rd day after operation (P < 0.01. On the contrary, the HGF level of HGF was lower in I / R + CD133 + kidney cell group than that in normal control group and I / R group (P <0.01). Compared with I / R + MSCs group, the levels of BUN and Cr, ATN and TNF-α in I / R + CD133 + kidney cell group were lower and HGF level was higher (P <0.05). Conclusion MSCs and CD133 + kidney cells may regulate the cytokines by endocrine, improve the microenvironment and promote the recovery of acute kidney injury induced by I / R. However, the repair effect of CD133 + kidney cells on acute kidney injury is more obvious.