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用离体人黄体细胞长期培养和放射免疫分析法观察肿瘤坏死因子-a(TNF-a)对孕酮分泌和cAMP生成的影响。结果表明:TNF-a显著抑制离体人黄体细胞基础孕酮分泌,TNF-a预处理24小时抑制其后4小时hCG诱导cAMP生成,其抑制作用具有显著的剂量依赖关系。
Effects of tumor necrosis factor-a (TNF-a) on progesterone secretion and cAMP production were observed by long-term culture in vitro and radioimmunoassay. The results showed that: TNF-a significantly inhibited the secretion of basal progesterone in human luteinized cells. Pretreatment with TNF-α for 24 hours inhibited hCG-induced cAMP production in the next 4 hours, and its inhibitory effect was significantly dose-dependent.