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目的掌握深圳市乙型肝炎病毒流行状况及基因型分布情况。方法对深圳市居民进行系统抽样,采用ELISA法检测乙肝表面抗原(HBs Ag),分析乙肝病毒感染状况。筛选出252份HBs Ag阳性血清提取DNA,采用荧光PCR方法进行HBV DNA定量检测及基因分型。结果乙肝表面抗原流行情况:共调查3 771人,深圳1岁~59岁人群HBs Ag阳性率为6.68%,低年龄组流行率显著低于高年龄组,差异有统计学意义(P<0.05)。基因分型选择83份DNA浓度≥1×103copy/ml的阳性标本进行检测,结果 B型检出率为73.5%,C型检出率为20.5%,B+C混合型检出率为1.2%,差异有统计学意义(P<0.05),无法分型4例(4.9%)。B、C基因型的分布在年龄、性别上差异无统计学意义(P>0.05)。C基因型感染者HBe Ag、抗-HBe Ag阳性率及HBV DNA水平均高于B基因型感染者,但差异无统计学意义(P>0.05)。结论深圳地区1岁~59岁人群中HBs Ag携带率低于全国平均水平,乙型肝炎人群中感染的HBV基因以B型为主,C型次之,B+C混合型最少。
Objective To understand the prevalence and genotype distribution of hepatitis B virus in Shenzhen. Methods Shenzhen residents were systematically sampled and tested for hepatitis B surface antigen (HBsAg) by ELISA, and the status of hepatitis B virus infection was analyzed. A total of 252 HBsAg-positive sera were screened out for DNA extraction. Fluorescent PCR was used to detect HBV DNA and genotype. Results The epidemic situation of HBsAg: a total of 3 771 persons were surveyed. The positive rate of HBsAg in children aged 1 to 59 years in Shenzhen was 6.68%, the prevalence of HBsAg in low age group was significantly lower than that of the high age group (P <0.05) . Genotyping 83 positive samples with DNA concentration ≥1 × 103copy / ml were selected for detection. The results showed that the detection rate of type B was 73.5%, the detection rate of type C was 20.5%, the detection rate of B + C mixed type was 1.2% , The difference was statistically significant (P <0.05), can not type 4 cases (4.9%). There was no significant difference in the distribution of B and C genotypes in age and sex (P> 0.05). The positive rate of HBe Ag, anti-HBe Ag and HBV DNA in patients with genotype C were higher than those with genotype B, but the difference was not statistically significant (P> 0.05). Conclusions The carrying rate of HBs Ag in Shenzhen population aged 1-59 years is lower than the national average level. The HBV genotypes in type B hepatitis B are mainly B type, followed by C type and B + C type.