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工频磁场是人类生活中接触最多的一类磁场,其引起的生物效应与人类健康的关系备受关注.本文选用1 mT、5 mT及10 mT工频磁场照射急性分离的小鼠皮层神经元(15 min),应用全细胞膜片钳技术离线记录通道电流,研究了工频磁场对神经元延迟整流钾通道特性的影响.结果显示,1 mT、5 mT及10 mT 3个强度的工频磁场对Ik均有抑制作用,但随着去极化电压的增加,发现1 mT和5 mT工频磁场的抑制率几乎不变,抑制率分别为(30±4.2)%和(20±2.2)%,而10 mT工频磁场的抑制率增加,最大抑制率为43.4%.另外,1 mT和5 mT工频磁场影响了延迟整流钾通道的激活特性,通道的半数激活电压变大,斜率因子不变.而10 mT工频磁场对通道的激活特性没有影响,半数激活电压和斜率因子均不改变.研究表明,工频磁场可能影响了细胞膜上离子通道蛋白质的结构和功能,并且不同强度工频磁场对通道的影响不同,存在强度窗口效应.
The frequency magnetic field is a kind of magnetic field with the most contact in human life, and the biological effect caused by it is closely concerned with the human health.In this paper, the acutely isolated mouse cortical neurons were irradiated with 1 mT, 5 mT and 10 mT frequency magnetic fields (15 min), the whole cell patch clamp technique was used to record the channel currents off-line, and the effect of power frequency magnetic field on the characteristics of delayed rectifier potassium channel in neurons was studied. The results showed that power frequency magnetic fields of 1 mT, 5 mT and 10 mT intensity However, with the increase of depolarization voltage, the inhibitory rates of 1 mT and 5 mT power frequency magnetic fields were almost unchanged, and the inhibitory rates were (30 ± 4.2)% and (20 ± 2.2)%, respectively , While the suppression rate of 10 mT power frequency magnetic field increased with the maximum inhibition rate of 43.4% .In addition, 1 mT and 5 mT power frequency magnetic field affected the activation characteristics of delayed rectifier potassium channel, half the activation voltage of the channel became larger, the slope factor was not While the 10 mT power frequency magnetic field has no effect on the activation characteristics of the channel, half of the activation voltage and the slope factor are not changed.Research shows that the power frequency magnetic field may affect the structure and function of ion channel proteins on the cell membrane, and different strength frequency The impact of the magnetic field on the channel, there Effect of the window.