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目的 :分析慢性前列腺炎对纤溶酶原激活因子 (PA)系统表达和活性的影响。 方法 :选取正常男性 2 3例 ,慢性前列腺炎患者 80例 (不育组和可生育组各 4 0例 )。采用纤维蛋白 琼脂糖PA指示胶打孔法和SDS PAGE电泳后指示胶铺盖法 ,测定精浆中总PA及组织型纤溶酶原激活因子 (tPA)和尿激酶型纤溶酶原激活因子 (uPA)的表达和活性。结果 :在正常人精浆中有高表达的总PA活性 ;而且同时表达tPA和uPA。慢性前列腺炎患者的精浆中总PA活性降低 ,tPA活性明显减低 ,uPA活性减低。慢性前列腺炎的可生育组和不育组精浆中总PA活性均降低 ,但两组间无明显差别。 结论 :患慢性前列腺炎时前列腺分泌功能降低 ,合成分泌PA减低。PA有可能在今后作为临床中判断前列腺功能检测指标之一
Objective: To analyze the effect of chronic prostatitis on the expression and activity of plasminogen activator (PA) system. Methods: Twenty-three normal men and 80 chronic prostatitis patients (40 infertile and 45 infertile) were selected. Fibrin Sepharose PA-indicating gel and SDS PAGE electrophoresis were used to instruct the method of plastic blanket to determine the total PA and serous plasminogen activator (tPA) and urokinase-type plasminogen activator uPA) expression and activity. Results: There was high expression of total PA activity in normal human seminal plasma; and both tPA and uPA were expressed. Chronic prostatitis in patients with seminal plasma total PA activity decreased, tPA activity was significantly reduced, uPA activity decreased. Chronic prostatitis fertile group and infertility group seminal plasma total PA activity were reduced, but no significant difference between the two groups. Conclusions: Prostate secretion is decreased and PA is decreased in patients with chronic prostatitis. PA may be used as one of the indicators of prostate function test in the future