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目的研究经大鼠尾静脉转染ｉＮＯＳ基因对大鼠血压的影响。方法从尾静脉注射经磷酸钙包裹的载有人类全长ｉＮＯＳｃＤＮＡ的表达质粒进入大鼠体内后，检测大鼠血压的改变及培养大鼠腹腔巨噬细胞上清液中ＮＯ的含量。结果（１）大鼠的血压从转染前的１８４．４７±７．８５ｍｍＨｇ，１６２．２３±１４．１３ｍｍＨｇ，于转染后第２天分别下降为１４５．２４±１３．４３ｍｍＨｇ（Ｐ＜０．０５，ｎ＝５）及１２８．９０±１５．０６ｍｍＨｇ（Ｐ＜０．０５，ｎ＝５），血压降低能维持６天以上；（２）转染ｉＮＯＳ基因使培养大鼠腹腔巨噬细胞上清液中ＮＯ水平由０．１２２±０．０１４ＯＤ值上升为０．２５４±０．０３１ＯＤ值３３（Ｐ＜０．０１，ｎ＝５，转染后第３天）及由０．１２５±０．０２１ＯＤ值上升为０．２０９±０．０１９ＯＤ值（Ｐ＜０．０１，ｎ＝５，转染后第６天）。结论静脉注射经磷酸钙包裹的ｉＮＯＳ基因进入大鼠体内，能成功地转染巨噬细胞，也可能转染其他具有吞噬功能的细胞，并表达其产物，使ＮＯ生成持续增加，导致血管舒张，血压降低，并在一段时间内维持较低水平。本实验为整体动物心血管系统的基因转染研究展示一种较为简便的方法
Objective To study the effect of transfection of iNOS gene on the blood pressure in rat tail vein. Methods The expression plasmid of human iNOS cDNA encapsulated with calcium phosphate was injected into rat tail vein from the tail vein to detect the change of blood pressure and the content of NO in the supernatant of rat peritoneal macrophages. Results (1) The blood pressure of rats decreased from 184.47 ± 7.85mmHg, 162.23 ± 14.13mmHg before transfection to 145.24 ± 13.43mmHg on the second day after transfection (P <0 .05, n = 5) and 128.90 ± 15.06mmHg (P <0.05, n = 5), blood pressure can be maintained for more than 6 days; (2) Transfection of iNOS gene to culture rat peritoneal macrophages The level of NO in the supernatant increased from 0.122 ± 0.014 OD to 0.254 ± 0.031 OD 33 (P <0.01, n = 5, day 3 after transfection) and from 0.125 ± 0.021 OD increased to 0.209 ± 0.019 OD (P <0.01, n = 5, 6 days after transfection). Conclusion Intravenous injection of calcium phosphate-coated iNOS gene into rats can successfully transfect macrophages or transfect other cells with phagocytic function and express their products, resulting in a sustained increase in NO production, resulting in vasodilation, Blood pressure drops and stays low for some time. This experiment shows a simpler method for gene transfection studies of the whole animal cardiovascular system