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目的 检查Ｂ．ａｂｏｒｔｕｓ３１ＫＤａ蛋白基因引物ＰＣＲ的特异性和敏感性。方法 用煮沸法和酚提法分别从６个种布鲁氏菌和耶氏菌Ｏ３、Ｏ９、大肠菌 Ｏ１５７．Ｈ７以及鼠伤寒沙门氏菌４７ ７２９中提取ＤＮＡ进行ＰＣＲ试验。结果３１ＫＤａ蛋白基因引物Ｂ４、Ｄ５对６个种布鲁氏菌ＤＮＡ均能扩增，对非布鲁氏菌ＤＮＡ呈阴性。该扩增反应与提取ＤＮＡ方法无关，与在一定范围内酶量关系不大。Ｂ４、Ｂ５引物的ＰＣＲ可查０．９５ｏｇ／μｌ的布鲁氏菌ＤＮＡ。结论Ｂ４、Ｂ５引物的ＰＣＲ具有良好的特异性和敏感性，可在现场中试用。
Purpose check B. Specificity and sensitivity of the abortus31KDa protein gene primer PCR. Methods Boiling and phenotyping were performed from six species of Brucella and Yersuies O3, O9 and Escherichia coli O157, respectively. H7 and Salmonella typhimurium 47 729 for PCR. Results 31KDa protein gene primers B4 and D5 could amplify the DNA of 6 species of Brucella and non-Brucella DNA negative. The amplification reaction has nothing to do with the method of DNA extraction, and has little to do with the amount of enzyme in a certain range. B4, B5 primer PCR check 0.95og / μl of Brucella DNA. Conclusion PCR of B4 and B5 primers has good specificity and sensitivity and can be tried in the field.