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本实验研究了ＮＢ４及ＨＬ－６０细胞的程序化死亡及细胞药代动力学特征。结果表明，安吖啶对ＮＢ４及ＨＬ－６０细胞均可诱导程序化死亡，但ＮＢ４细胞较ＨＬ－６０细胞更敏感，形态学改变更明显；ＤＮＡ凝胶电泳梯带更明显。安吖啶作用后，两株细胞的胞膜完整性可保持２４－４８ｈ。与４ｍｇ／Ｌ的安吖啶孵温后，非同步化细胞内安吖啶浓度在６ｈ达到高峰（ＮＢ４和ＨＬ－６０细胞分别为２５７．３９±３．２１ｎｇ／１０７细胞和１７４．９６±８．７６ｎｇ／１０７细胞），随后逐渐降低。但安吖啶诱导两株细胞程序化死亡的细胞数都随时间延长而逐渐增加。在３，６，１２ｈ时ＮＢ４细胞内药物浓度高于ＨＬ－６０细胞（Ｐ＜０．０１），Ｇ２Ｍ期的ＮＢ４细胞内的药物浓度较ＨＬ－６０细胞高（Ｐ＜０．０５）。安吖啶诱导程序化死亡作用与细胞内药物蓄积有关，并存在时间上的延迟反应
This study investigated the programmed death and cellular pharmacokinetics of NB4 and HL-60 cells. The results showed that ampicillin could induce programmed death in both NB4 and HL-60 cells, but NB4 cells were more sensitive than HL-60 cells, and the morphological changes were more obvious; DNA gel electrophoresis ladders were more obvious. After ampicillin treatment, the cell membrane integrity of both cells can be maintained for 24-48 h. After incubating with 4 mg/L amsacride, the unsynchronized intracellular ampicillin concentration peaked at 6 h (NB4 and HL-60 cells were 257.39±3.21 ng/107 cells and 174.96±8, respectively. .76 ng/107 cells), then gradually decreased. However, the number of cells that ampicillin induced the programmed death of both cells gradually increased with time. At 3, 6, and 12 h, the concentration of NB4 cells was higher than that of HL-60 cells (P<0.01). The concentration of NB4 cells in G2M phase was higher than that of HL-60 cells (P<0.05). Ampicillin-induced programmed death associated with intracellular drug accumulation and delayed reaction in time