论文部分内容阅读
目的:探讨不同剂量三七总皂苷预处理后对大鼠肾缺血再灌注损伤NF-κB信号通路的影响。方法:SD大鼠40只,随机分为空白对照组,肾缺血再灌注损伤组,三七总皂苷40、80、160 mg/kg组,前两组给予生理盐水,后3组分别给予三七总皂苷40、80、160 mg/kg连续给药5天。后4组在最后1次给药1h后通过切除右肾、夹闭左肾动脉45min再灌注3h的方法制备肾缺血再灌注损伤模型。术后处死大鼠,检测血肌酐、尿素氮,HE染色观察肾组织病理学改变,免疫组化法和PT-PCR法分别检测核因子-κBp65、白细胞介素-1β蛋白水平和mRNA表达。结果:肾缺血再灌注损伤组BUN、SCr水平明显增高,光镜下可见肾小管损伤明显,肾组织NF-κBp65、IL-1β蛋白水平和mRNA表达明显增高。三七总皂苷40、80、160 mg/kg组BUN、SCr水平明显降低,肾组织病理学改变明显减轻,NF-κBp65、IL-1β蛋白水平和mRNA表达显著降低,其中三七总皂苷160 mg/kg组的效果最明显。结论:三七总皂苷可能通过抑制肾缺血再灌注损伤肾组织中NF-κBp65、IL-1β的表达,减轻氧化应激损伤,从而对肾组织起到有效的保护作用。
Objective: To investigate the effects of different doses of Panax Notoginseng saponins preconditioning on NF-κB signaling pathway in rat renal ischemia-reperfusion injury. Methods: Forty SD rats were randomly divided into blank control group, renal ischemia-reperfusion injury group, Panax notoginseng saponins 40,80,160 mg / kg group, the first two groups were given normal saline, the latter three groups were given three Seven total saponins 40,80,160 mg / kg for 5 days. The rats in the latter 4 groups were given ischemia reperfusion injury model by removing the right kidney and clamping the left renal artery for 45 min and reperfusion for 3 h after the last administration for 1 h. The rats were killed after operation, serum creatinine and urea nitrogen were detected. The pathological changes of renal tissue were observed by HE staining. The expressions of nuclear factor-κBp65, interleukin-1β and mRNA were detected by immunohistochemistry and PT-PCR respectively. Results: The levels of BUN and SCr in renal ischemia / reperfusion injury group were significantly increased. The damage of renal tubules was obvious under light microscope. The protein level and mRNA expression of NF-κBp65 and IL-1β in renal tissue were significantly increased. The levels of BUN and SCr in 40, 80 and 160 mg / kg groups of Panax notoginseng saponins were significantly decreased, the pathological changes of renal tissues were significantly reduced, the protein levels of NF-κBp65, IL-1β and mRNA expression were significantly decreased, of which Panax notoginseng 160 mg / kg group the most obvious effect. Conclusion: Panax notoginseng saponins may play an effective protective effect on renal tissues by inhibiting the expression of NF-κBp65 and IL-1β in renal tissues after renal ischemia-reperfusion injury and reducing oxidative stress injury.