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目的:探讨紫心甘薯总黄酮(PSPF)对D-半乳糖致衰老小鼠学习记忆能力的影响及对小鼠脑组织氧化损伤的保护作用。方法:ICR小鼠随机分为6组(n=10):正常组(NC),模型组,维生素C组(100 mg.kg-1.d-1),PSPF低、中、高(50,100,200 mg.kg-1.d-1)剂量组。除正常组外,小鼠颈背部皮下注射D-半乳糖1 g.kg-1.d-1连续42 d,建立亚急性衰老模型,从造模第2周开始分别灌胃给予各组相应药物。42 d后采用Morris水迷宫法测定衰老小鼠学习记忆能力,同时测定小鼠脑组织丙二醛(MDA)含量、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-PX)活性。结果:与模型组相比,PSPF中、高剂量组能明显改善小鼠的衰老体征,缩短逃避潜伏期和空间搜索实验中第一次到达平台所在区域的时间(P<0.05),增加穿越平台所在区域的次数(P<0.05,P<0.01),并能有效提高衰老小鼠脑组织SOD和GSH-PX活性,降低MDA含量(P<0.05,P<0.01),呈现量效依赖关系。结论:PSPF能改善D-半乳糖致衰老小鼠的学习记忆能力,具有延缓衰老的作用,其作用机制可能与机体抗氧化酶活性增加,清除自由基、抗氧化能力提高所致。
Objective: To investigate the effects of purple heart sweet potato flavonoids (PSPF) on learning and memory in aging mice induced by D-galactose and its protective effect on oxidative damage in mice brain. Methods: ICR mice were randomly divided into 6 groups (n = 10): normal group (NC), model group, vitamin C group (100 mg.kg-1.d-1), PSPF low, middle and high kg-1.d-1) dose group. In addition to the normal group, mice subcutaneous injection of D-galactose 1 g.kg-1.d-1 subcutaneously on the back of the neck for 42 consecutive days to establish sub-acute aging model, . After 42 days, the learning and memory abilities of aging mice were measured by Morris water maze method. The levels of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH- PX) activity. Results: Compared with the model group, the middle and high doses of PSPF could significantly improve the aging signs of mice, shorten the escape latency and the time of the space search experiment reaching the region where the platform was located for the first time (P <0.05) (P <0.05, P <0.01), and could effectively increase the activity of SOD and GSH-PX in brain tissue of aging mice and decrease the content of MDA (P <0.05, P <0.01), showing a dose-dependent relationship. CONCLUSION: PSPF can improve the learning and memory ability of aging mice induced by D-galactose and has the effect of delaying aging. The mechanism may be related to the increase of antioxidant enzyme activity, free radical scavenging and antioxidative abilities.