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本文以小鼠骨髓细胞（ＢＭＣ）和经条件化筛选的分化程度较一致的小鼠骨髓巨噬细胞（ＢＭＭ）为研究对象，摸索了用ＭＴＴ法测定人巨噬细胞集落刺激因子（ｈＭ－ＣＳＦ）生物活性的最佳条件。结果显示ＢＭＣ细胞的线性检测范围为６００～２５００ＣＦＵ／ｍｌ；ＢＭＭ细胞的线性检测范围明显改善，为１５０～１２５０ＣＦＵ／ｍｌ。该法的建立与改善提供了简便、可靠、适于检测大批量Ｍ－ＣＳＦ样品生物活性的方法。
In this study, mouse bone marrow cells (BMCs) and mouse bone marrow macrophages (BMMs) with the same degree of differentiation were screened by MTT method. The effects of hM-CSF ) The best conditions for biological activity. The results showed that the linear detection range of BMC cells was 600 ~ 2500CFU / ml; the linear detection range of BMM cells was significantly improved, which was 150 ~ 1250CFU / ml. The establishment and improvement of this method provided a simple, reliable and suitable method for detecting the biological activity of high-volume M-CSF samples.