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银耳孢子(Tremella fuciformis Berk.spores),多糖(简称TF)的急性和亚急性毒性试验表明毒性很小,小鼠腹腔注射LD_(50)为1696.68±135.64mg/kg。狗静脉注射前后,血象、肝、肾功能以及组织切片均未见有由于注射TF而引起的不正常或病理性变化和损伤。腹腔注射银耳孢子多糖可促进溶血素的产生,其半数溶血值比对照组增加3.10倍,并能增加小鼠脾脏的重量,脾指数增加0.56倍。体外实验表明,TF可增加E玫瑰花结的形成率,形成率增加1.08倍,TF还可使大鼠红细胞凝集,凝集率达60.(?)％,TF对巨噬细蜘胞的吞噬功能有促进作用,吞噬指数和百分率分別为对照组的2.6和1.5倍,对淋巴细胞DNA和RNA的生物合成也有促进作用,3H-TdR和3H-UR掺入量分別增加0.75和1.11倍,此外,TF对~(60)Coγ射线所致的损伤有保护作用存活率为对照组的1.78倍对由于环磷酰胺中毒所引起的白细胞下降也有明显的对抗作用,并且有明显促进血清蛋白生物合成的作用。前已报道有关TF的提取、分离、纯化和理化分析。本文报导了TF的生物活性及其对机体毒性的初步研究。
The acute and subacute toxicity tests of Tremella fuciformis Berk.spores and polysaccharides (referred to as TF) showed that the toxicity was very low. The LD_ (50) of mice injected intraperitoneally was 1696.68 ± 135.64mg / kg. Before and after the intravenous injection of dog, there were no abnormal or pathological changes and injuries caused by the injection of TF in blood, liver, kidney function and tissue sections. Intraperitoneal injection of Tremella spores polysaccharide can promote the production of hemolysin, half of its hemolytic value increased 3.10 times than the control group, and can increase the weight of mouse spleen, spleen index increased 0.56 times. In vitro experiments showed that TF can increase the formation rate of E rosette, the formation rate increased 1.08 times, TF can also make rat erythrocyte agglutination, the agglutination rate of 60 (?)%, TF macrophage fine phagocytosis The phagocytosis index and percentage were 2.6 and 1.5 times that of the control group, respectively, which also promoted the biosynthesis of DNA and RNA in lymphocytes. The incorporation of 3H-TdR and 3H-UR increased 0.75 and 1.11 times respectively. In addition, TF has a protective effect on the damage induced by ~ (60) Coγ-rays The survival rate of 1.78 times as much as that of the control group is also significantly antagonistic to the leukopenia caused by cyclophosphamide poisoning, and significantly promote the role of serum protein biosynthesis . Previous reports on the TF extraction, separation, purification and physical and chemical analysis. This article reports on the biological activity of TF and its preliminary study on the toxicity of TF.