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本文观察了经异种免疫核糖核酸致后的小鼠脾细胞内cAMP和cGMP水平的变化,拟从环型核苷酸代谢的角度为免疫敏核糖核酸作用机制提供资料。实验结果表明,小鼠脾淋巴细胞与正常脾核糖核酸或免疫核糖核酸保温后,细胞内cAMP含量均有增加,此种增加在正常脾RNA组与免疫RNA组之间无明显差异。而免疫RNA可使小鼠脾淋巴细胞内cGMP含量在保温后30′明显增高,正常脾RNA则无此作用。细胞毒实验证明,免疫RNA传递细胞介导细胞毒功能与其增高小鼠脾淋巴细胞内cGMP水平之间有一致关系,两种生物活性均对核糖核酸酶敏感,表明它们均有赖于保持免疫RNA的大分子完整性。
In this paper, the changes of cAMP and cGMP levels in mouse spleen cells induced by xeno-immunosorbent assay (RNAi) were investigated. The mechanism of immunosensin-ribonucleic acid (RNAi) function was proposed from the perspective of circular nucleotide metabolism. The experimental results show that the splenic lymphocytes and normal spleen ribonucleic acid or immune ribonucleic acid after incubation, intracellular cAMP content increased, this increase in normal spleen RNA group and immune RNA group no significant difference. The immune RNA can make mouse splenic lymphocyte cGMP content significantly increased 30 ’after incubation, normal spleen RNA has no such effect. Cytotoxicity experiments show that the immune-mediated cell-mediated cytotoxicity and its increased splenic lymphocytes in mouse cGMP levels have a consistent relationship between the two biological activity are ribonuclease sensitive, indicating that they all rely on the maintenance of immune RNA Macromolecule integrity.