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应用毛细管区带电泳（ＣＺＥ）分析测定阿片受体、抗独特型抗羟甲芬太尼抗体等生物大分子样品。熔融石英毛细管柱为６０ｃｍ×１００μｍｉ．ｄ．（从进样到检测器长度为５０ｃｍ），以硼砂－氢氧化钾为缓冲液。结果表明缓冲液的ｐＨ影响ＣＺＥ对阿片受体的分析，当ｐＨ为９．０时分析效果最理想，重现性良好，测得的两个主峰与同批样品进行ＳＤＳ－ＰＡＧＥ分析得到的两个条带相符。用ＣＺＥ分析抗独特型抗羟甲芬太尼抗体（ＩｇＧ），表明用ＰｒＯｔｅｉｎＡ－Ｓｅｐｈａｒｏｓｅ亲和色谱分离得到部分纯化的ＩｇＧ，迁移时间不同于对照豚鼠的ＩｇＧ。
Application of capillary zone electrophoresis (CZE) analysis of opioid receptors, anti-idiotypic anti-hydroxy-fentanyl antibodies and other biological macromolecules samples. The fused silica capillary column was 60 cm × 100 μm. d. (From injection to detector length of 50cm) to borax - potassium hydroxide buffer. The results showed that the pH of the buffer affected the analysis of opioid receptors by CZE. When the pH value was 9.0, the analytical result was the best and the reproducibility was good. The two main peaks obtained by SDS-PAGE analysis with the two main peaks obtained A match. Anti-idiotypic anti-hydroxy-fentanyl antibody (IgG) was analyzed by CZE, indicating that partially purified IgG was isolated by PrOtein A-Sepharose affinity chromatography at a time different from that of the control guinea pig.