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用低浓度ＰＥＧ（ＭＷ６０００）提取的日本血吸虫感染兔血清中循环免疫复合物（ＣＩＣ）直接免疫家兔，制备抗ＣＩＣ兔血清。采用酶联免疫印迹技术（ＥＩＴＢ）分析鉴定日本血吸虫ＣＩＣ中抗原成份。结果表明，ＣＩＣ中成虫及其排泄分泌物源性抗原成份有１７种，虫卵源性抗原成份有５种。用ＩＦＡＴ方法分析ＣＩＣ中抗原成份定位于成虫表膜及肠腔上皮。
Anti-CIC rabbit serum was prepared by immunizing rabbits with circulating immunocomplex (CIC) of Schistosoma japonicum infected rabbits extracted with low concentration of PEG (MW6000). The antigenic components of Schistosoma japonicum CIC were identified by enzyme-linked immunosorbent assay (EITB). The results showed that there were 17 kinds of antigenic components in adults and excretion secretions of CIC, and 5 kinds of components of egg-derived antigen. Analysis of antigens in CIC by IFAT method was performed on the adult epidermis and intestinal lumen.