光肩星天牛是危害非常严重的林业蛀干害虫之一,光肩星天牛幼虫鉴定相对困难,而在进出口木质包装中截获的虫态大多为幼虫或蛹,因此对其快速而准确的鉴定十分重要。本文通过对包括光肩星天牛在内的天牛科6种常见危险性林业有害生物线粒体DNA的COI基因序列比对分析,设计并筛选出一对特异性引物,该引物的PCR检测方法可直接应用于光肩星天牛幼虫的鉴定,这为我国进出境检验检疫工作提供了技术支持。 Anoplophora glabripennis is one of the most harmful insect pests in forestry. The identification of larvae of Anoplophora glabripennis is relatively difficult. However, it is very important to identify the larvae or pupae in larvae or pupae in the import and export of wood packaging. In this paper, we have designed and screened a pair of specific primers for COI gene sequence analysis of mitochondrial DNA of 6 kinds of common pests of Cerambycidae including Apostichopus japonicus. The PCR detection method of this primer can be directly applied to the genetic diversity of Asteraceae Larvae identification, which for China’s entry and exit inspection and quarantine provided technical support.