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Objective To investigate the electrochemiluminescence (ECL) behavior of cloperastine hydrochloride. Methods ECL intensity of tris (2,2′-bipyridyl) rutheniumo(Ⅱ) was enhanced, the method for the determination of cloperastine hydrochloride was established using capillary electrophoresis (CE) coupled with electrochemilumolinescence (ECL) detection. Results Under the optimum conditions, ECL intensity varied linearly with cloperastine hydrochloride concentration from 7.0×10-6g/mL to 1.0×10-4g/mL. The detection limit (S/N=3) was 8.05×10-7g/mL. The relative standard deviation of the ECL intensity and the migration time for 11 consecutive injections of 1.0×10-5g/mL cloperastine hydrochloride was 2.9% and 1.5%, respectively. This method was successfully applied to cloperastine hydrochloride tablet determination. Conclusion The method has been established, validated and applied for determination of cloperastine hydrochloride.
Methods To determine the cloperastine hydrochloride was established using capillary electrophoresis (CE) coupled Results of the optimal conditions, ECL intensity varied linearly with cloperastine hydrochloride concentration from 7.0 × 10-6 g / mL to 1.0 × 10-4 g / mL. The detection limit (S / N = 3) was 8.05 × 10-7g / mL. The relative standard deviation of the ECL intensity and the migration time for 11 consecutive injections of 1.0 × 10-5g / mL cloperastine hydrochloride was 2.9% and 1.5%, respectively. This method was successfully applied to cloperastine hydrochloride tablet determination. Conclusion The method has been established, validated and applied for determination of cloperastine hydrochloride.