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肿瘤微转移是分子诊断分期的重要依据 ,是指导治疗的重要参考。检测非小细胞肺癌 (NSCLC)微转移 ,可明确术前肿瘤分期 ,制定合理治疗方案 ;根据术中微转移可指导术后辅助治疗 ;检测外周血中肿瘤细胞及转移灶变化可以指导化疗 ;微转移灶的监测还可判断预后。检测微转移的方法包括常用的免疫组化法、逆转录PCR技术、流式细胞技术以及不常用的细胞培养方法等。
Micrometastasis is an important basis for the molecular diagnosis of staging, is an important reference to guide the treatment. Detection of non-small cell lung cancer (NSCLC) micrometastasis, can be clear preoperative tumor staging, to develop a reasonable treatment options; according to intraoperative microtransfer can guide postoperative adjuvant therapy; detection of peripheral blood tumor cells and metastasis changes can guide chemotherapy; micro Metastasis monitoring can also determine the prognosis. Methods for detecting micrometastases include commonly used immunohistochemical methods, reverse transcription PCR techniques, flow cytometry techniques, and infrequently used cell culture methods.