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2007年4月,烟台国际旅行卫生保健中心采用ELISA法对出入境人员进行艾滋病感染初筛时,发现1份HIV抗体检测结果为强阳性血清。随即将阳性血清送山东国际旅行卫生保健中心采用ELISA法复检,结果仍为强阳性;采用免疫印迹法(WB)进行确认,结果出现gp160、p24双条带。然而在对受检者进行流行病学调查时,其强烈否认有高危性行为,怀疑检测结果的可靠性。随后,阳性血清被送往北京国际旅行卫生保健中心艾滋病确证中心实验室采用免疫印迹法进行疑难确证,结果同样出现gp160、p24双条带。进一步用核酸定量检测(NASBA)进行HIV载量检测,结果为阴性。因此,建议作为HIV抗体阴性不确定进行随访。后经1年的多次随访检测,最终排除了其HIV感染。上述情况表明,对WB检测结果仅出现gp160和p24条带的样本直接判定为阳性具有一定的风险,必须结合核酸检测等辅助检测方法和流行病学调查结果综合考虑,艾滋病实验室在出具检测结果报告时,一定要慎重。
In April 2007, Yantai International Travel Health Care Center used ELISA to detect HIV infection among immigration officers. One HIV antibody test result showed strong positive serum. Then positive serum sent to Shandong International Travel Health Care Center by ELISA retest, the result is still strong positive; confirmed by Western blot (WB), the results appear gp160, p24 double band. However, during the epidemiological investigation of the subjects, it strongly denied having high-risk sexual behaviors and doubted the reliability of the test results. Subsequently, the positive serum was sent to the Beijing International Travel Health Care Center of AIDS confirmed the use of immunoblotting laboratories difficult confirmation, the results also appeared gp160, p24 double band. Further detection of HIV load with NASBA was negative. Therefore, we recommend follow-up as an HIV antibody negative uncertainty. After a year of multiple follow-up testing, eventually ruled out its HIV infection. The above situation shows that there is a certain risk to the WB test result that the samples with only gp160 and p24 bands appear to be positive and must be combined with the auxiliary detection methods such as nucleic acid test and the results of epidemiological investigation. When reporting, we must be careful.