论文部分内容阅读
目的:介绍一种建立兔颞下颌关节囊内黏连动物模型的方法。方法:手术暴露16只兔右侧的颧突根部、眶下壁和眶后壁处组织,在颧突根部的前下方,用带有弹力橡皮筋的针自下向上垂直穿过关节盘的前伸部,将双股弹力橡皮筋从9.5mm向前牵引至40mm,达1.20N的牵引力,并将其固定于眶骨上制备的小孔中。术后分期进行MRI和内镜检查,确定有、无关节盘移位和黏连形成,随后处死动物取关节标本,以备光镜、电镜研究。结果:所有兔的下颌骨均不同程度地偏向对照侧,且伴有前牙的斜向磨耗。MRI证实,所有关节盘均出现了前移位,部分动物模型的实验侧关节上腔内有黏连带形成。结论:本实验提供了一种较好的建立颞下颌关节囊内黏连动物模型的方法。
OBJECTIVE: To introduce a method to establish an animal model of rabbit temporomandibular joint adhesions. Methods: The left zygomatic root, the infraorbital wall and the retro orbital wall of the right rabbits of 16 rabbits were surgically exposed. The anterior and inferior sides of the root of the zygomaticus were exposed by the needles with elastic bands vertically from bottom to top Stretch, the bifurcated elastic rubber band from 9.5mm forward traction to 40mm, 1.20N of traction, and its fixation in the orbital bone prepared holes. MRI and endoscopy were performed after the operation to determine the presence or absence of coherence disc displacement and adhesion formation. Then, the animals were sacrificed and the joint specimens were sacrificed for preparation by light microscope and electron microscope. Results: All rabbits mandibles were biased toward the control side to varying degrees with diagonal wear of the anterior teeth. MRI confirmed the presence of anterior shift in all of the articular discs and the formation of adhesions in the superior articular cavity of some animal models. Conclusion: This experiment provides a better method to establish animal models of temporomandibular joint adhesions.