用灌注法从实验室感染鼠收集曼氏血吸虫成虫(PR-1株)。灌注液用含0.1%葡萄糖和0.5%水解乳蛋白的欧氏平衡盐溶液(EBSS)。灌注后立即将虫体移到冰槽内盛有新鲜EBSS的小指管内。将合抱成虫和灌注后分开的雌、雄虫分3批分别立即固定,在4℃,5%CO_2条件下温浴半小时、1小时后固定,冰冻切片。固定液为10%中性福尔马林缓冲液(NBF)。处死实验鼠,取出内脏置于NBF固定半小时后,将含虫体的肠系膜 Adult Schistosoma mansoni (PR-1 strain) was collected from laboratory infected mice by perfusion. The perfusate was treated with an Ellis-balanced salt solution (EBSS) containing 0.1% glucose and 0.5% hydrolyzed milk protein. Immediately after perfusion, the parasites were moved into the small vial containing fresh EBSS in the ice tray. The female and male insects separated from each other after the entrapment and perfusion were fixed in 3 batches immediately. After being incubated at 4 ° C and 5% CO 2 for half an hour, they were fixed after 1 hour and frozen and sliced. Fixation was 10% neutral formalin buffer (NBF). The mice were sacrificed, and the viscera was removed and fixed in NBF for half an hour before the worms contained the mesentery