论文部分内容阅读
根据ｓＩＬ－６Ｒ的结构特点及其功能分析，利用ＰＣＲ技术选择性地扩增两个不同长度的ＩＬ－６ＲｃＤＮＡ胞外片段。经ＤＮＡ测序和Ｓｏｕｔｈｅｒｎｂｌｏｔ证实后，定向插入原核表达载体ｐＥＴ－３ａ中，获得了中、高效表达。
According to the structural characteristics and functional analysis of sIL-6R, two extracellular fragments of IL-6R cDNA were selectively amplified by PCR. After confirmed by DNA sequencing and Southern blot, the prokaryotic expression vector pET-3a was inserted into prokaryotic expression vector pET-3a to obtain medium and high expression.