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将受检者血清免疫复合物（CIC）解离后，以抗-SEAPcAb作ELISA特异性检测非游离性循环抗原（NF－CAg）。通过30例不同病期患者血清、已治疗血吸虫病人血清、其它寄生虫病患者血清和健康人血清的对比检测结果表明，急性、慢性和晚期血吸虫病人的阳性率分别为76.7％、80.0％和60．0％，且三者的NF－CAg水乎（OD均值）均显著高于健康者（P均＜0．01），其它寄生虫病患者和健康人的交叉反应率和假阳性率分别为5．0～10.0％和8.8％，前者检测的NF－CAg水平，均接近健康入水平。经治疗后3个月、6个月及3年的血吸虫病入血清检测结果转阴率分别为86．7％、82．0％和96．7％，且其NF－CAg水平（OD值），均接近或低于健康人的水平。上述结果进一步表明，血吸虫病人血清CIC解离后特异性检测NF－CAg具有较好的诊断病人和考核疗效的价值。
After dissociation of the serum immunocomplex (CIC) of the subject, anti-SEAPcAb was used as an ELISA to specifically detect the non-circulating antigens (NF-CAg). Sera from 30 patients with different stages of disease, serum from treated schistosomiasis patients, serum from other parasitic diseases and healthy human serum showed that the positive rates of acute, chronic and advanced schistosomiasis were 76.7%, 80.0% and 60 (P <0.01). The rates of cross-reaction and false-positive of other parasitic diseases and healthy people were 5.0 ~ 10.0% and 8.8%, the former detection of NF-CAg levels, are close to the healthy into the level. The results showed that the negative conversion rates of schistosomiasis into serum at 3 months, 6 months and 3 years after treatment were 86.7%, 82.0% and 96.7% respectively, and the levels of NF-CAg (OD) , Are close to or below the level of healthy people. The above results further show that the specific detection of NF-CAg after schistosomiasis serum CIC dissociation has better diagnostic value of patients and assessment of efficacy.