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应用特异性单克隆抗体作DOT-ELISA和竞争ELISA检测65例脑囊虫病患者血清和脑脊液中游离循环Ag(FCAg)和循环免疫复合物抗原(CICAg)。血清FCAg阳性率分别为81.3%和81.5%(0.20~87.0μg/ml),CICAg阳性率为95.4%和92.3%(0.19~120.0μg/ml),脑脊液测定FCAg阳性单为78.5%和86.2%(0.32~25.0μg/ml),CICAg阳性率为90.8%和93.8%(0.19~46.4μg/ml)。结果表明检测总循环抗原(包括游离部分和复合物部分)有利于提高脑囊虫病的诊断。
Serum and cerebrospinal fluid (CSF) and circulating immunocomplex antigen (CICAg) were detected in 65 patients with cysticercosis using DOT-ELISA and competitive ELISA. The positive rates of FCAg in serum were 81.3% and 81.5% (0.20 ~ 87.0μg / ml), the positive rates of CICAg were 95.4% and 92.3% (0.19 ~ 120.0μg / ml) The positive rates were 78.5% and 86.2% (0.32 ~ 25.0μg / ml). The positive rates of CICAg were 90.8% and 93.8% (0.19 ~ 46.4μg / ml) respectively. The results show that the detection of total circulating antigen (including the free part and the complex part) is helpful to improve the diagnosis of cerebral cysticercosis.