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用Rep-PCR和RFLP两种方法分析了143个水稻白叶枯病Xanthomonas oryzae pv.oryzae菌系。这些菌系采集于长江流域,华南,华北和东北的20个省市的96个点。Rap-PCR是利用一些基于细菌的短的重复单位引物(ERIC,BOX和REP)及来自稻白叶枯病原菌的可动重复单位(IS1112和IS1113)引物的DNA扩增特性。在RFLP分析中,以无毒基因家族中的一个成员avrXa10为探针。Rep-PCR分析呈显较高的单元型多样性(H=0.75)。在4个水稻生长地区中,Rep-PCR分析的结果是:华南稻区的遗传多样性最高(H=0.96),随后为长江流域(H=0.93),东北(H=0.77),华北(H=0.69)。根据3个分簇生物统计的共有指纹型,143个菌系被构成3簇。在各簇中都有来自不同水稻产区的菌系,表明菌系的分簇不受地理区域的影响。
143 strains of Xanthomonas oryzae pv.oryzae were analyzed by using two methods of Rep-PCR and RFLP. These strains were collected at 96 sites in 20 provinces and cities in the Yangtze River Valley, South China, North China and Northeast China. Rap-PCR utilizes the DNA amplification characteristics of some bacterial-based short repeat unit primers (ERIC, BOX and REP) and primers from the mobile repeat units (IS1112 and IS1113) of the rice leaf blight. In RFLP analysis, avrXa10, a member of the avirulent gene family, was used as a probe. Rep-PCR analysis showed a higher haplotype diversity (H = 0.75). The results of the Rep-PCR analysis showed that the highest genetic diversity (H = 0.96) in the rice growing areas of South China was found in the four rice growing regions, followed by the Yangtze River valley (H = 0.93), northeast = 0.69). Based on the three fingerprinting biometrics, 143 strains were grouped into three clusters. Strains from different rice producing areas are present in each cluster, indicating that the colonies of the strains are unaffected by the geographical area.