目的 :建立泛昔洛韦人体内活性代谢产物—喷昔洛韦血浆浓度测定方法。方法 :采用C18反相色谱柱 ,以0 0 2mol·L-1高氯酸 -甲醇 (4 5 0∶8)为流动相 ,荧光检测波长为λex=2 70nm ,λem=375nm ,内标物为阿昔洛韦 ,血浆样品采用 6mol·L-1高氯酸液沉淀蛋白。结果 :喷昔洛韦血浆浓度在 0 12 5～ 16mg·L-1范围内 ,与面积呈良好的线性关系 (r=0 9998) ;本试验最低检测浓度为 0 0 5mg·L-1,日内及日间RSD均小于 8% ,喷昔洛韦的方法回收率大于 90 %。结论 :该方法简便、准确、灵敏 ,可用于泛昔洛韦人体药代动力学及生物利用度的研究 Objective: To establish a method for the determination of plasma concentration of penciclovir, the active metabolite of famciclovir in human body. Methods: C18 reversed-phase column was used. The mobile phase consisted of 0 0 2 mol·L-1 perchloric acid-methanol (4 5 0:8). The detection wavelength was λex = 2 70nm, λem = 375nm. Acyclovir, plasma samples using 6mol · L-1 perchloric acid precipitation of protein. Results: The plasma concentration of penciclovir ranged from 0 12 5 to 16 mg · L-1, and showed a good linear relationship with the area (r = 0 9998). The lowest concentration of penciclovir was 0 0 5 mg · L-1 in days And daytime RSDs were less than 8%, penciclovir method recovery was greater than 90%. Conclusion: The method is simple, accurate and sensitive and can be used to study the pharmacokinetics and bioavailability of famciclovir