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研究出一种适合农村基层小型工厂化育苗的简便的香蕉快速繁殖生产技术程序。将香蕉吸芽茎尖接种在以MS为基本培养基上,附加BA4毫克/升、NAA0.5毫克/升,为芽增殖率最高培养茎;附加KT0.2毫克/升、NAA0.1毫克/升,为促根最适培养基。接种后的茎尖和芽增殖阶段采用黑暗培养,黑暗培养比光培养增殖速度提高20-30％。芽增殖周期缩至20天,增殖系数为3。芽长至2-3厘米便可转移到促根培养基上,利用自然光照,温度保持在20-30℃,经20天便培养成健壮的完整植株,移植到土镶中,成活率达90％以上。
Developed a simple and suitable for rural grassroots small plant breeding rapid banana rapid breeding production technology program. The banana shoots were inoculated on MS basal medium supplemented with BA4 mg / L and NAA 0.5 mg / L, the highest stems with the highest shoot proliferation rate; the additional KT0.2 mg / L and NAA0.1 mg / L, to promote root optimum medium. After inoculation, the shoot tips and buds proliferated in darkness, and the proliferation rate in darkness was 20-30% higher than that in light culture. Bud proliferation cycle reduced to 20 days, proliferation coefficient of 3. Buds grow to 2-3 cm can be transferred to promote rooting medium, the use of natural light, the temperature maintained at 20-30 ℃, after 20 days to grow into a robust whole plant, transplanted into soil set, the survival rate of 90 %the above.