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目的研究宫颈癌中脆性组氨酸三联体(FHIT)基因特定位点的单核苷酸多态性表达。方法选定FHIT基因上位点,针对其分别设计特异性引物,做聚合酶链反应生成目的条带,应用Pyro Mark ID DNA遗传分析系统做SNP的分析。结果 rs17255497、rs1385816、rs114014393个位点的PCR成功扩增。其中rs17255497位点基因型均为G/G型。rs1385816位点基因型为A/A型占48.9%,A/G型占40%,G/G型占11.1%,实验组、对照组无显著性差异。rs11401439位点基因型未能判断。结沦rs17255497位点汉族中国人基因型均为G/G型。rs1385816位点基因型为A/A型占48.9%,A/G型占40%,G/G型占11.1%,实验组、对照组无显著性差异。rs11401439位点基因型未能判断,可能产生丢失或插入,引起后面序列的编码改变。
Objective To study the single nucleotide polymorphism (SNP) expression of a specific site of the FHIT gene in cervical cancer. Methods The specific sites of FHIT gene were selected and specific primers were designed respectively. The PCR products were amplified by polymerase chain reaction (PCR) and analyzed by Pyro Mark ID DNA genetic analysis system. Results PCR amplification of rs17255497, rs1385816, rs114014393 loci was successful. The rs17255497 locus genotypes were G / G type. The genotypes of rs1385816 were A / A 48.9%, A / G 40% and G / G 11.1%. There was no significant difference between the experimental group and the control group. rs11401439 locus genotype failed to judge. Conclusions rs17255497 locus Han Chinese genotypes are G / G type. The genotypes of rs1385816 were A / A 48.9%, A / G 40% and G / G 11.1%. There was no significant difference between the experimental group and the control group. rs11401439 locus genotype failed to judge, may be lost or inserted, causing subsequent coding sequence changes.