目的探讨血小板中钙离子感受蛋白基质相互作用因子1(STIM1)蛋白在急性缺血性脑卒中的表达以及其与脑梗死发作严重程度和预后的相关性。方法回顾性分析本院收治的120例急性缺血性脑梗死患者外周血和相关临床资料,分离外周血中血小板,通过western blotting检测患者血小板内STIM1蛋白含量。使用NIHSS评分标准判断急性缺血性脑卒中患者发病24小时内病情严重程度,并在三个月后对病人进行随访,重新评估病人NIHSS评分以判断患者预后情况。运用统计分析方法分析健康人以及病情严重程度不同的急性脑梗死患者血小板内STIM1蛋白表达含量的差异是否存在统计学意义,并分析其与缺血性中风短期预后的相关性。结果在患病人群中其平均STIM1蛋白表达量比正常人群表达量有明显升高(P<0.01)。患病严重程度不同的病人之间,其外周血血小板内STIM1蛋白表达量存在差异,并且外周血血小板内STIM1蛋白表达量与病人的患病严重程度存在正相关的关系。结论血小板STIM1蛋白表达增加提示患者具有更严重的脑卒中,其三个月预后差。这种作用可能是因为血小板内STIM1蛋白含量一定程度反映了体内血小板的活性状态,临床应紧密检测该指标,尽早治疗以防止病情进一步恶化。 Objective To investigate the expression of platelet factor STIM1 in acute ischemic stroke and its relationship with the severity and prognosis of cerebral infarction. Methods The peripheral blood and related clinical data of 120 patients with acute ischemic cerebral infarction admitted to our hospital were retrospectively analyzed. The platelets in peripheral blood were separated and the content of STIM1 protein in platelets was detected by western blotting. The NIHSS score was used to determine the severity of the disease within 24 hours of the onset of acute ischemic stroke and the patient was followed up three months later to re-evaluate the NIHSS score to determine the patient’s prognosis. Statistical analysis was used to analyze the difference of STIM1 protein expression in healthy volunteers and acute cerebral infarction patients with different severity of illness whether there was statistical significance or not, and the correlation between STIM1 protein and short-term prognosis of ischemic stroke was analyzed. Results The mean STIM1 protein expression in the affected population was significantly higher than that in the normal population (P <0.01). STIM1 protein expression in peripheral blood platelets varies between patients with different severity of illness, and there is a positive correlation between the expression of STIM1 protein in peripheral blood platelets and the patient’s severity of illness. Conclusion The increased expression of platelet STIM1 protein suggests that patients have more severe stroke and their prognosis is poor at three months. This effect may be due to platelet STIM1 protein content to some extent reflects the in vivo activity of platelets, the clinical test should be closely monitored, as soon as possible to prevent further deterioration of the disease.