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为观察体内电转染对HBV基因免疫诱导的特异性体液免疫应答的调节作用,将HBV-preS2/S编码基因插入pVAON33载体构建重组质粒pVAON33-preS2/S,运用肌肉注射法对BALB/c小鼠进行基因免疫(100ug质粒DNA.100ul.只)。以pVAON33-preS2/S、pVAON33-preS2/S(体内电转染)为实验组,并以pVAON33空质粒为对照。按期采集免疫小鼠血清。采用ELISA法检测免疫小鼠血清特异性抗HBs-IgG抗体。结果显示,pVAON33-preS2/S免疫小鼠后可诱导产生特异性抗HBs-IgG抗体,到第7周时其OD值为0.73±0.18(P/N为2.13),而pVAON33-preS2/S(体内电转染)组诱导了更高水平的特异性抗HBs-IgG抗体,第7周时OD值为1.30±0.45(P/N为3.79),两组比较有显著性差异(P<0.05)。本研究表明体内电转染可明显增强HBV基因免疫诱导的体液免疫应答。
In order to observe the regulatory effect of electroporation in vivo on the specific humoral immune response induced by HBV gene immunity, HBV-preS2 / S gene was inserted into pVAON33 vector to construct recombinant plasmid pVAON33-preS2 / S. Mice were immunized (100 ug plasmid DNA. PVAON33-preS2 / S, pVAON33-preS2 / S (in vivo electroporation) as experimental group, and pVAON33 empty plasmid as a control. Immune mouse sera were collected on schedule. Serum specific anti-HBs-IgG antibody was detected by ELISA. The results showed that pVAON33-preS2 / S immunized mice could induce specific anti-HBs-IgG antibodies, with the OD value of 0.73 ± 0.18 (P / N of 2.13) at the 7th week, while pVAON33-preS2 / S (P / N = 3.79). There was a significant difference between the two groups (P <0.05). At the seventh week, the OD value was 1.30 ± 0.45 (P / N = 3.79) . This study shows that in vivo transfection can significantly enhance humoral immune response induced by HBV gene immunity.