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目的观察转化生长因子β1(TGF-β1)对人胃癌SGC7901细胞增殖和凋亡的影响,探讨其可能的作用机制。方法使用不同浓度的TGF-β1(0.5、1、2、5、ng·m L-1)干预人胃癌细胞SGC7901不同时间(6、12、24、48、72h),通过MTT法检测细胞生长抑制率,流式细胞仪检测细胞凋亡情况,RT-PCR法检测细胞内Smad3、Smad7 m RNA的表达水平。结果 (1)在0.5~10 ng·m L-1的浓度范围内,TGF-β1以剂量依赖性方式抑制胃癌SGC7901细胞增殖,并表现出明显的量效关系(r=0.908,P<0.01);且随着作用时间的延长,TGF-β1对胃癌SGC7901细胞的抑制率逐渐升高,呈时间依赖性。5 ng·m L-1及10 ng·m L-1TGF-β1作用于胃癌SGC7901细胞72 h后,生长抑制率分别为(61.12±0.98)%、(63.67±0.18)%(与无TGF-β1处理组相比,P<0.01),抑制作用相当。(2)5 ng·m L-1 TGF-β1作用于人胃癌SGC7901细胞不同时间(0、6、12、24、48、72 h)后,细胞凋亡率没有明显改变(P>0.05),分别为1.32%、2.61%、3.15%、3.31%、3.76%、3.82%;10 ng·m L-1 TGF-β1作用于人胃癌SGC7901细胞不同时间(0、6、12、24、48、72 h)后,细胞凋亡率没有明显改变(P>0.05),分别为1.88%、2.57%、1.57%、1.67%、2.04%、4.58%。(3)0.5~10 ng·m L-1 TGF-β1作用于人胃癌SGC7901细胞时,TGF-β1以剂量依赖性方式增加胃癌SGC7901细胞Smad3、Smad7的m RNA表达(与无TGF-β1处理组相比,P<0.01)。结论 TGF-β1可以浓度和剂量依赖性方式抑制胃癌SGC7901细胞的增殖,这可能与其增加smad3和smad7的m RNA表达有关,但TGF-β1并不能促进胃癌SGC7901细胞的凋亡。
Objective To investigate the effect of transforming growth factor β1 (TGF-β1) on the proliferation and apoptosis of human gastric cancer cell line SGC7901 and to explore its possible mechanism. Methods Human gastric cancer cell line SGC7901 was treated with different concentrations of TGF-β1 (0.5, 1, 2, 5, ng · m L -1) for different time periods (6,12,24,48,72h), and cell growth inhibition The cell apoptosis was detected by flow cytometry. The expression of Smad3 and Smad7 mRNA in the cells was detected by RT-PCR. Results (1) In the concentration range of 0.5 ~ 10 ng · m L-1, TGF-β1 inhibited the proliferation of SGC7901 cells in a dose-dependent manner and showed a significant dose-response relationship (r = 0.908, ; And with the extension of time, the inhibitory rate of TGF-β1 on gastric cancer SGC7901 cells gradually increased in a time-dependent manner. The growth inhibition rates of SGC7901 cells treated with 5 ng · m L -1 and 10 ng · m L -1 TGF-β1 for 72 h were (61.12 ± 0.98)%, (63.67 ± 0.18)% Treatment group compared to P <0.01), the same inhibition. (2) The apoptosis rate of SGC7901 cells treated with 5 ng · m L-1 TGF-β1 at different times (0, 6, 12, 24, 48 and 72 h) did not change significantly (P> 0.05) (1.32%, 2.61%, 3.15%, 3.31%, 3.76%, 3.82%, respectively). The effect of 10 ng · m L-1 TGF-β1 on human gastric cancer SGC7901 cells at different times (0,6,12,24,48,72 h), the apoptotic rates did not change significantly (P> 0.05), which were 1.88%, 2.57%, 1.57%, 1.67%, 2.04%, 4.58% respectively. (3) TGF-β1 increased the mRNA expression of Smad3 and Smad7 in SGC7901 cells in a dose-dependent manner when treated with 0.5 ~ 10 ng · m L -1 TGF-β1 (compared with TGF-β1-treated group Compared to P <0.01). Conclusion TGF-β1 can inhibit the proliferation of SGC7901 cells in a concentration-and dose-dependent manner, which may be related to the increase of smad3 and smad7 mRNA expression. However, TGF-β1 does not promote the apoptosis of gastric cancer SGC7901 cells.