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材料和方法一、临床资料:患者30人,从1989年9月至1990年4月到我院门诊就诊的病人,其中轻型RAU20人,重型7人,口炎型3人。男性14人,女性16人,年龄从19～65岁。对照组30人,为健康献血员,男性18人,女性12人,年龄20～45岁。受试者空腹抽血4ml,不抗凝,分别作叶酸和维生素B_(12)放射免疫测定。二、测定方法1 叶酸测定:被检血清加入一定量的~(123)I—叶酸及叶酸抗体,35℃保温30分钟,使之进行竞争结合,加聚乙二醇及载体蛋白,离心使结合的~(125)I—叶酸沉淀(B),吸弃
Materials and methods First, the clinical data: 30 patients, from September 1989 to April 1990 to our hospital outpatient treatment patients, including 20 light-duty RAU, 7 were heavy, stomatitis type 3. 14 males and 16 females, aged from 19 to 65 years old. Control group of 30 people, healthy blood donors, 18 males and 12 females, aged 20 to 45 years. Subjects fasting blood 4ml, non-anticoagulant, respectively, for folic acid and vitamin B_ (12) radioimmunoassay. Second, the determination of 1 folic acid test: serum was seized by adding a certain amount of ~ (123) I-folic acid and folic acid antibodies, 35 ℃ incubated for 30 minutes to make competitive combination, add polyethylene glycol and carrier protein, centrifugation to make the combination ~ (125) I-folic acid precipitate (B), Absorbed