土霉素片是我国药典(1977年版)收载的最常用药物制剂之一,我国《兽药规范》(1978年版)也收载有土霉素原料,它们的含量均是用抗生素微生物检定法来测定。根据文献记载,其0.1N 硫酸溶液,在269nm、320nm 和352nm 波长处有最大吸收。本文选择352nm 波长对土霉素片进行含量测定。先用标准品测绘出标准曲线,求出工作方程,然后根据被测样品的吸收度,即可计算出它的含量。本法操作简易,测定回收率较好,结果准确,是测定土霉 Oxytetracycline tablets is one of the most commonly used pharmaceutical preparations contained in the Chinese Pharmacopoeia (1977 edition). Our country’s “Code of Veterinary Medicine” (1978 Edition) also contains oxytetracycline raw materials, their content is determined by antibiotic microbial test Determination. According to the literature, the 0.1 N sulfuric acid solution has maximum absorption at 269 nm, 320 nm and 352 nm wavelengths. This article selected 352nm wavelength oxytetracycline tablets content determination. First standard curve with a standard curve, work out the equation, and then based on the absorption of the sample was measured, you can calculate its content. The method is simple, the determination of recovery is better, the result is accurate, is the determination of mold