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目的:采用高效液相色谱法测定不同产地野生滇龙胆中龙胆苦苷、獐牙菜苦苷及獐牙菜苷的含量。方法:岛津Shim-pack VP-ODS柱(4.6 mm×150 mm,5μm),流动相甲醇-水(30∶70),流速1.0 mL·min-1,龙胆苦苷检测波长270 nm,獐牙菜苦苷及獐牙菜苷检测波长245 nm,柱温25℃。结果:龙胆苦苷、獐牙菜苦苷、獐牙菜苷的线性范围为0.4～2 mg·L-1;相关系数分别为0.999 7,0.999 6,0.998 7;回收率分别为100.04%(RSD 1.49%),98.48%(RSD 1.67%),98.46%(RSD 1.15%)结论:用本法测定不同产地野生滇龙胆中3种成分含量,方法可行,结果可靠,为野生滇龙胆资源的开发与利用提供资料。
OBJECTIVE: To determine the contents of gentiopicroside, swertiarin and swertia glycoside in Gentiana yunnanensis Gentiana in different habitats by high performance liquid chromatography (HPLC). Methods Shimadzu Shim-pack VP-ODS column (4.6 mm × 150 mm, 5 μm), mobile phase methanol-water (30:70), flow rate 1.0 mL · min-1, gentiopicroside detection wavelength 270 nm, Carnosine and swertia glycosides detection wavelength 245 nm, column temperature 25 ℃. Results: The linear ranges of gentiopicroside, swertiarin and swertiadenin were 0.4-2 mg · L-1, the correlation coefficients were 0.999 7, 0.999 6 and 0.998 7, respectively. The recovery rates were 100.04% RSD 1.49%), 98.48% (RSD 1.67%) and 98.46% (RSD 1.15%) respectively. Conclusion: The method can be used to determine the content of three components in Gentiana yunnanensis from different habitats. The method is reliable and the results are reliable. The development and utilization of information to provide.