论文部分内容阅读
目的:观察早期肾透明细胞癌及癌旁0.5、1.0、2.0cm组织上皮膜抗原(epithelial membrane antigen,EMA)、肾细胞癌标志物(renal cell carcinoma marker,RCC-Ma)的表达差异,初步探讨早期肾癌行肾部分切除术时的安全切除边距。方法:构建包含44例早期肾透明细胞癌及癌旁0.5、1.0、2.0cm肾皮质的组织芯片,采用免疫组化染色分别观察癌及癌旁(距肿瘤边缘0.5、1.0、2.0cm)组织EMA、RCC-Ma的表达差异。结果:EMA、RCC-Ma在癌及癌旁组织均为细胞膜和(或)胞质染色。癌组织EMA和RCC-Ma强阳性表达率明显高于癌旁组织各组(15.91%/18.18%vs84.09%/79.55%、86.36%/77.27%、79.55%/75.00%,P<0.01);而癌旁组织各组(距肿瘤边缘0.5、1.0、2.0cm)间差异无统计学意义(P>0.05)。结论:0.5cm可能是早期肾透明细胞癌腹腔镜肾部分切除术安全的切除边距。
OBJECTIVE: To observe the expression difference of epithelial membrane antigen (EMA) and renal cell carcinoma marker (RCC-Ma) in early stage renal clear cell carcinoma and adjacent tissues of 0.5, 1.0 and 2.0 cm. Early renal cell carcinoma underwent partial resection of the margin of safety margins. Methods: Tissue microarrays containing 44 cases of early renal clear cell carcinoma and 0.5, 1.0 and 2.0 cm of renal cortex adjacent to cancer were constructed. Immunohistochemical staining was used to observe the expression of EMA in cancer and paracancerous tissues (0.5, 1.0 and 2.0 cm away from tumor margin) , RCC-Ma expression differences. Results: EMA and RCC-Ma were both in plasma membrane and / or cytoplasm in both cancer and paracancerous tissues. The positive rates of EMA and RCC - Ma in cancerous tissues were significantly higher than those in adjacent normal tissues (15.91% / 18.18% vs84.09% / 79.55%, 86.36% / 77.27%, 79.55% / 75.00%, P <0.01). However, no significant difference was found between adjacent tumor tissues (0.5, 1.0 and 2.0 cm away from tumor margin) (P> 0.05). CONCLUSIONS: 0.5 cm may be a safe margin of resection for laparoscopic partial nephrectomy of early stage clear cell renal cell carcinoma.