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目的:观察心脉龙注射液(XML)对大鼠缺氧及缺氧-复氧心肌细胞[Ca2+]及丙二醛(MDA)、超氧化物歧化酶(SOD)的影响,以进一步阐明其作用机制。 方法:采用Fura-2/AM及相应试剂盒测定该药对大鼠缺氧及缺氧-复氧心肌细胞|Ca2+|i,MDA和SOD的影响。结果:7.5×102μg/mL XML能升高缺氧及缺氧-复氧心肌细胞|Ca2+|i(P<0.01,P<0.05),此作用与去乙酰毛花苷相当3.75 × 102,1.5×103μg/mL XML则略降低心肌细胞|Ca2+|i(P>0.05),同时应用维拉帕米均不能抑制去乙酰毛花苷及XML各剂量组对细胞内|Ca2+|i的影响。 3.1 ×102,6.3×102,1.25×103μg/mL XML能升高缺氧及缺氧-复氧心肌细胞悬液的SOD含量(P<0.01,P<0.05),虽能降低MDA,但无统计学意义 结论:对缺氧及缺氧-复氧状态的衰竭心脏,在强心时要严格掌握XML的剂量,其强心作用与心肌细胞|Ca2+|i的升高有关,该药同时还具有一定的抗氧化作用。
Objective: To observe the effects of Xinmailong injection (XML) on hypoxia and [Ca2+] and malondialdehyde (MDA) and superoxide dismutase (SOD) in rat hypoxic and reoxygenative cardiomyocytes to further elucidate Mechanism. METHODS: Fura-2/AM and corresponding kits were used to determine the effect of the drug on hypoxia and hypoxia-reoxygenation myocardial cells, Ca2+, i, MDA and SOD. RESULTS: 7.5×102μg/mL XML could up-regulate hypoxia and hypoxia-reoxygenation cardiomyocytes |Ca2+|i (P<0.01, P<0.05). This effect was comparable to that of acetylated lanatoside 3.75 × 102, 1.5× 103μg/mL XML slightly decreased the myocardial cells |Ca2+|i(P>0.05), while the application of verapamil could not inhibit the effect of deacetyllactoside and XML dose on intracellular Ca2+|. 3.1 × 102, 6.3 × 102, 1.25 × 103μg/mL XML can increase SOD content in hypoxic and hypoxic-reoxygenated cardiomyocyte suspension (P <0.01, P <0.05), although it can reduce MDA, but no statistics Significance of the study Conclusions: For hypoxic and hypoxic-reoxygenated failing hearts, the dose of XML must be strictly controlled when the heart is strong. Its cardiac effect is related to the elevation of myocardial cells, Ca2+|, which also has Certain antioxidant effect.