论文部分内容阅读
建立了用高效液相色谱法同时测定制马钱子中士的宁和马钱子碱的含量。氯仿－浓氨溶液（９０∶１０）作溶剂，超声波振荡提取。硅胶柱，氯仿－甲醇－浓氨溶液（９０∶１０∶０．３）为流动相，紫外２５４ｎｍ波长检测。平均加样回收率（ｎ＝５）士的宁为９８．２％，ＲＳＤ＝１．４％；马钱子碱为９９．３％，ＲＳＤ＝３．３％。日内、日间精密度（ＲＳＤ）士的宁分别为０．６％和１．３％；马钱子碱分别为１．１％和２．４％。
Simultaneous determination of strychnine and strychnine in Strychnos nux vomica by high performance liquid chromatography was established. Chloroform - concentrated ammonia solution (90:10) as solvent, extracted by ultrasonic oscillation. Silica gel column, chloroform - methanol - concentrated ammonia solution (90:10:0.3) as the mobile phase, UV 254nm wavelength detection. The average recovery rate (n = 5) was 98.2% for strychnine, RSD = 1.4%; strychnine was 99.3% with RSD = 3.3%. Day and day RSD strychnine were 0.6% and 1.3% respectively; brucine was 1.1% and 2.4% respectively.