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目的 :研究慢性乙型肝炎(chronic hepatitis B,CHB)患者体内白细胞介素(interleukin,IL)-37的表达情况及其临床意义。方法:收集38例CHB患者(CHB组)和11名健康志愿者(对照组)的血液标本,采用反转录聚合酶链反应法及蛋白印迹法检测2组患者外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)中IL-37 m RNA和相应蛋白的表达水平;并应用酶联免疫吸附试验检测2组血清中的IL-37水平,比较2组间的差异。结果:与对照组相比,CHB组PBMCs中IL-37 m RNA和蛋白的表达均明显降低;而对照组血清中IL-37浓度为(36.3±5.2)pg/m L,CHB组为(22.2±1.4)pg/m L,2组间差异有统计学意义(P=0.012)。结论 :IL-37参与了CHB的发病过程,本研究可能为CHB患者的治疗提供新思路。
Objective: To study the expression of interleukin-37 (IL-37) in patients with chronic hepatitis B (CHB) and its clinical significance. Methods: Blood samples from 38 CHB patients (CHB group) and 11 healthy volunteers (control group) were collected. Reverse transcription polymerase chain reaction and Western blotting were used to detect peripheral blood mononuclear cells (peripheral blood mononuclear cells (PBMCs)). The levels of IL-37 mRNA and protein in the two groups were detected by enzyme linked immunosorbent assay (ELISA), and the differences between the two groups were compared. Results: Compared with control group, the expression of IL-37 mRNA and protein in PBMCs of CHB group was significantly lower than that of control group, while the level of IL-37 in control group was (36.3 ± 5.2) pg / m L and that of CHB group was (22.2 ± 1.4) pg / m L, the difference between the two groups was statistically significant (P = 0.012). Conclusion: IL-37 participates in the pathogenesis of CHB, and this study may provide new ideas for the treatment of CHB patients.