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应用细胞融合技术建立抗人ＩｇＧ４ＭｃＡｂ杂交瘤细胞，生产ＭｃＡｂ。以此为探针，应用ＥＬＩＳＡ方法检测班氏微丝蚴血症血清中特异ＩｇＧ４的阳性率为９５．９１％（４７／４９）；治疗后的原微丝蚴血症ＩｇＧ４阳性率为１．４５％（１／６９）；７７例晚期丝虫病症状者及５０份非流行区健康人血清均为阴性；肠道线虫感染、囊虫感染及华支睾吸虫感染者血清均未出现交叉反应；检测原班氏丝虫病高、中度流行区正常人群滤纸干血的特异ＩｇＧ４阳性率分别为０．７９％（６／７６０）和０．２７％（３／１０９５）。显示特异ＩｇＧ４检测具有较高的敏感性与特异性。是诊断丝虫感染的有效方法，可取代微丝蚴血检用于现场的人群丝虫病监测。
Anti-human IgG4McAb hybridoma cells were established by cell fusion technique to produce McAb. Using this as a probe, the positive rate of specific IgG4 in the serum of Bancrofti microfilariae was 95.91% (47/49) by ELISA. The positive rate of IgG4 in the original microfilaremia after treatment was 1. 45% (1/69); 77 cases of late filariasis symptoms and 50 non-endemic areas of healthy human serum were negative; intestinal nematode infection, cysticercosis and Clonorchis sinensis infection did not cross-react serum The positive rates of specific IgG4 in filter paper dried blood of high and moderate endemic areas were 0.79% (6/760) and 0.27% (3/1095), respectively. Specific IgG4 detection showed a higher sensitivity and specificity. Is an effective method of diagnosis of filarial infection can replace the microfilaria blood test for the crowd in the field of filariasis monitoring.